The implementation of information technology in healthcare sector is growing due various great benefits offered by its usage. This study investigates IT profiles of Malaysian small, medium and enterprise (SME) private healthcare as well as personnel perception towards Health Information System (HIS) usage in their organization. A conceptual framework of factors affecting intention to use HIS is developed by adapting the technology acceptance model and the theory of planned behavior focusing on specific dimensions - individual, technological and implementation. This study uses survey that involves 252 respondents from SME private healthcare in Malaysia. A descriptive analysis results of SME private healthcare IT profile, user perceptions of HIS and the developed conceptual framework are presented in this paper together with the structural equation modeling analysis result of the proposed model.